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Биохимическая адаптация лекарственных растений к температурному режиму как биологический индикатор уровня стресса

Сведения об участнике
ФИО
Живетьев Максим Аркадьевич
ФИО (на английском языке)
Zhivetev Maksim Arkadievish
Название организации
Siberian Institute of Plant Physiology and Biochemistry Siberian Branch of RAS
Тезисы
Название
Биохимическая адаптация лекарственных растений к температурному режиму как биологический индикатор уровня стресса
Название (на английском языке)
Medicinal herb biochemical adaptation to thermal regimes as a biological indicator of stress levels
Соавторы (ФИО, организация, город, страна)
Граскова Ирина Алексеевна, СИФИБР СО РАН, Иркутск, Россия
Соавторы (на английском языке)
Graskova Irina Alekseevna, SIPPB SB RAS, Irkutsk, Russia
Содержание

Our objects of research were leaves from three species medicinal herbs, Achlleaasiatica (Serg.), Taraxacumofficinale (Wigg.), and Pantago major (L.). Each species grow in the city of Irkutsk and the southeastern shore of Lake Baikal. In leaves of each species, we sought to identify activity of peroxidase, fatty acid content, sugar accumulation, and stress-induced protein production. In autumn, sugar accumulation, stress-induced protein production, and unsaturated fatty acid content were registered higher in Lake Baikal plants than their Irkutsk counterparts, suggesting an adaptation complex of Baikal herbs. These mechanisms can be used as a biological indicator of stress levels for Baikal plants in their native habitat.

Keywords: medicinal herbs, Achillea asiatica, Taraxacum officinale, Plantago major, adaptation, natural conditions, peroxidase activity, stress-induced proteins

 

The research sought to assess the stats of both Lake Baikal and the surrounding area as an indirect means to analyze climatic influences. In this attempt, we measured the effect of low temperatures on protein, lipid, and carbohydrate metabolisms by measuring certain signal molecules, including active oxygen forms and peroxidase activity.

For this research, our main objects were three,medicinally herbal species, Achillea asiatica (Serg.), Taraxacum officinale (Wigg.), and Plantago major (L.), that occur naturally within Irkutsk and the southeastern shore of Lake Baikal. In leaves of each species, we measured peroxidase activity, fatty acid content, sugar accumulation, and stress-induced protein production.

Variation in cell wall associated peroxidase as well as soluble peroxidase was identified as dependent upon both the reigning thermal regime and location of growth [1].

With impending negative temperatures, peroxidase activity within Irkutsk-dwelling plants decreased in October. Inversely, for plants located at Lake Baikal, peroxidase activity increased.

The described phenomena can be described as an adaptive mechanism for plants in Irkutsk and the Baikal shore in response to environmental stimuli.

Thermostability and optimal pH of cell wall associated and soluble peroxidases were shown to change in relation to natural habitat location and

season of the year [2]. Additionally, peroxidase activity was variable throughout the vegetation period.

When in a condition of increased hydrogen peroxide presence, a logical increase in peroxidase was observed, preceded by dehydrine expression [3].

An increase in sugar content, functioning as a cryoprotective mechanism, was observed towards the end of the vegetative season [4]. Sugar accumulation in plants on the shores of Lake Baikal was observed to contain lower concentrations than Irkutsk counterparts.

In conclusion, sugar accumulation, stress-induced protein production [5], and unsaturated fatty acid content [6], existing within the leaves of Baikal flora, were noticed higher at Lake Baikal than in Irkutsk. By regularly measuring these biochemical characteristics, the system can be used as system of ecological standing for Baikal plants.

 

Literature

1. Zhivetyev M.A., Graskova I.A., Voinikov V.K. Activity of guaiacol-dependent peroxidase in Plantago major L. leaves // Journal of Stress Physiol.& Biochemistry. 2013. Vol. 9. I. 3. P. 326-332.

2. Zhivetyev M.A., Graskova I.A., Voinikov V.K. Dependence of guaiacol peroxidase activity on pH in officinal plant leaves // Journal of Stress Physiol.& Biochemistry. 2013. Vol. 9. I. 1. P. 283–288.

3. Zhivet’ev M.A., Rachenco E.I, Putilina T.E., Krasnobaev V.А., Graskova I.A., Voinikov V.K. Activity and isoenzyme spectrum of peroxidases and degydrins of some plant species, growing on the shores of Lake Baikal, under abiotic stress // Journal of Stress Physiol.& Biochemistry, 2010. Vol. 6. I. 4. P. 42-50.

4. Zhivetev M.A., Graskova I.A., Pomortsev A.V., Voinikov V.K. Water and carbohydrate content at leafs of plants used in medicine during vegetation // Journal of Stress Physiology & Biochemistry. 2011. Vol. 7. I. 4. P. 69-79.

5. Zhivetiev M.A., Kolesnichenko A.V., Graskova I.A., Voinikov V.K. Seasonal Dynamics of stress proteins in leaves of medicinal plants in a natural environment of Irkutsk and on the shores of the Lake Baikal // Journal of Stress Physiol.& Biochemistry. 2014. Vol. 10. I. 4. P. 42-50.

6. Zhivet’ev M.A., Graskova I.A., Dudareva L.V., Stolbikova A.V., Voinikov V.K. Change of fatty-acid composition in plants during adaptation to hypothermia // Journal of Stress Physiol.& Biochemistry. 2010. Vol. 6. I. 4. P. 51-65.

Благодарности
Acknowledgments: I should like to thank the following individuals for their support and contribution with this work. Michael Meyer, US National Science Foundation Fellow and PhD student, from Washington State University. Dudareva Lubov Vissarionovna, chief of laboratory physical-chemical methods of analysis, Siberian Institute of Plant Physiology and Biochemistry Siberian Branch of RAS.