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Chlorophyll fluorescence parameters of various plant (moss Anthoceros and pepperwort-salad) for environment quality estimation

Сведения об участнике
ФИО
Пикуленко Марина Маиловна
ФИО (на английском языке)
Pikulenko Marina M.
Название организации
Mосковский Государственный университет имени М. В. Ломоносова
Информация о докладе
Вид доклада
Постерный доклад
Секция
Биоиндикация и химический анализ в экологическом мониторинге
Название доклада
Chlorophyll fluorescence parameters of various plant (moss Anthoceros and pepperwort-salad) for environment quality estimation
Соавторы доклада (ФИО, организация, город, страна)
Булычев А.А.; МГУ имени М. В. Ломоносова, г. Москва, Россия
Аннотация
The monitoring of ecological characteristics of biological systems may include the measuring of the physiological parameters of the cells organelles and the membrane complexes. In the case green plants, analysis of chlorophyll fluorescence provides considerable information about primary reactions of photosynthesis, including the efficiency of charge separations in photosystem II (PSII), the rate of linear electron transport, and “energy-dependent quenching” related to the pH gradient (∆pH). In addition to ∆pH, the transmembrane electric potential of thylakoids is crucial for energy conversion. The choice of a hornwort Anthoceros is substantiated by such advantages as the feasibility of simultaneous measurements of the chloroplast membrane potential, the electric potential changes at the cell membrane, and changes in chlorophyll fluorescence parameters occurring upon the absorption of photosynthetically active radiation (PAR). Its thin thallus facilitates the access of substances from the outer medium to the cell surface, which is important for testing the action of physiologically active agents on photopotentials of the chloroplast and cell membranes. The use of small-size thalli necessitates the application of sensitive fluorometric devices collecting light emission from the area of 0.01 to 10 mm2. Specialized fluorometers - a Microscopy-PAM (Walz, Germany) based on the saturation pulse method and a plant efficiency analyzer (PEA, Hansatech, United Kingdom) designed for measurements of the fluorescence induction curves with a time resolution of 10-6s - fully meet these requirements. The Microscopy-PAM device allows measurements on microscopic parts of a thallus such parameters as the quantum yield of the charge separations in PSII (∆F/Fm’), coefficient of photochemical quenching related to the redox state of PSII primary acceptor, and the coefficient of nonphotochemical quenching indicative of energy-dependent fluorescence quenching related to ∆pH formation in the thylakoids. So we suggest for environmental impact assessment as a test-objects the moss Anthoceros and 10-day pepperwort-salad seedlings simultaneously. Integrated impact assessment of adverse conditions on the parameters of photosynthetic activity of moss Anthoceros is perspective, despite the difficult regulatory relationship between photosynthesis and membrane processes. Study of fluorescence parameters of pepperwort-salad for estimation the oil pollution of water in terms of salinity showed nonspecific reaction.
Ключевые слова
enviromental monitoring, biotesting, parameters of photosynthetic activity, test-object the moss Anthoceros
Введение
Не заполнено
Методы и материалы
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Полученные результаты
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Заключение
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Цитируемая литература
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Благодарности
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Название, авторы, резюме (на английском языке)

Chlorophyll fluorescence parameters of various plant (moss Anthoceros and pepperwort-salad) for environment quality estimation 

Pikulenko Marina M., Bulechev Alexander A.

The monitoring of ecological characteristics of biological systems may include the measuring of the physiological parameters of the cells organelles and the membrane complexes. In the case green plants, analysis of chlorophyll fluorescence provides considerable information about primary reactions of photosynthesis, including the efficiency of charge separations in photosystem II (PSII), the rate of linear electron transport, and “energy-dependent quenching” related to the pH gradient (∆pH). In addition to ∆pH, the transmembrane electric potential of thylakoids is crucial for energy conversion. The choice of a hornwort Anthoceros is substantiated by such advantages as the feasibility of simultaneous measurements of the chloroplast membrane potential, the electric potential changes at the cell membrane, and changes in chlorophyll fluorescence parameters occurring upon the absorption of photosynthetically active radiation (PAR). Its thin thallus facilitates the access of substances from the outer medium to the cell surface, which is important for testing the action of physiologically active agents on photopotentials of the chloroplast and cell membranes. The use of small-size thalli necessitates the application of sensitive fluorometric devices collecting light emission from the area of 0.01 to 10 mm2. Specialized fluorometers - a Microscopy-PAM (Walz, Germany) based on the saturation pulse method and a plant efficiency analyzer (PEA, Hansatech, United Kingdom) designed for measurements of the fluorescence induction curves with a time resolution of 10-6s - fully meet these requirements. The Microscopy-PAM device allows measurements on microscopic parts of a thallus such parameters as the quantum yield of the charge separations in PSII (∆F/Fm’), coefficient of photochemical quenching related to the redox state of PSII primary acceptor, and the coefficient of nonphotochemical quenching indicative of energy-dependent fluorescence quenching related to ∆pH formation in the thylakoids.  So we suggest for environmental impact assessment as a test-objects the moss Anthoceros and 10-day pepperwort-salad seedlings simultaneously. Integrated impact assessment of adverse conditions on the parameters of photosynthetic activity of moss Anthoceros is perspective, despite the difficult regulatory relationship between photosynthesis and membrane processes. Study of fluorescence parameters of pepperwort-salad for estimation the oil pollution of water in terms of salinity showed nonspecific reaction.