In vivo Visualization of Nanocomposite Microcapsules Addressed by Magnetic Field Gradient
One of the fast developing directions of research in the nanomedicine area is optical imaging in vivo. The common method of visualization enables carry out bioluminescent and fluorescent imaging for biological processes. This is method used for looking at biodistribution by significantly amount reducing of required animals and increasing of experimental data for each animals in interest region. Polyelectrolyte microcapsules have interest as potential containers for drug delivery due to ease of preparation, multifunctionality and sensitivity to external influence. Microcapsules have a number advantages, such as high loading efficiency, possibility for load a magnetic nanoparticles and fluorescent substances into a capsule which allows to track and control of microcapsules in vivo. We described to using composite microcapsules for research a distant control in vivo by magnetic field gradient. The visualization of microcapsules in vivo was on stage by Near-Infrared Fluorescence Imaging for Real-Time. The microcapsules contain magnetite nanoparticles (FeNP) and fluorescent dye – Cyanine 7 NSH-ester conjugating with bovine serum albumin (BSA-Cy7) and polyarginine (pArg). The average size of microcapsule was about 5±1μm and 0.7±0.1μm. The microcapsule suspension injections into tail vein (systemic administration) and into femoral artery (local administration) were proposed out respectively. The magnetic targeting of microcapsules with average size 0.7±0.1μm for femoral artery injection is more efficient compare with tail significantly higher comparison tail vein injection was established. This approach open new possibilities for targeted drugs delivery system.