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New research directions in the Nizhny Novgorod State Medical Academy on the basis of the megagrant achivements

Name
Elena
Surname
Zagaynova
Scientific organization
Nizhny Novgorod State Medical Academy
Academic degree
Doctor of science, Professor RAS
Position
Director of Institute of Biomedical Technologies
Scientific discipline
Life Sciences & Medicine
Topic
New research directions in the Nizhny Novgorod State Medical Academy on the basis of the megagrant achivements
Abstract
In 2010-2014 the project "Fluorescent proteins: novel approaches to the study of the mechanisms of physiological and pathological processes in living systems" was successfully completed. The project focused on the development and use of new approaches to the study of the molecular mechanisms of biological processes and targeted effects on living systems using the latest generation of fluorescent markers. Laboratory of fluorescence bioimaging successfully continuous work in the field of fluorescent proteins for cancer and stem cells research, and supported by new grants.
Keywords
Fluorescent proteins, geneticallyencoded sensors for pH, H2O2, metabolic imaging, anticancer drug discovery
Summary

In 2010 the Nizhny Novgorod State Medical Academy (NNSMA) won the first megagrant competition, organized by the Government of the Russian Federation (Project № 11.G34.31.0017). As a result, a leading scientist Academician Sergey Anatolyevich Lukyanov organized a new Laboratory of fluorescence bioimaging in NNSMA.

In 2010-2014 the project "Fluorescent proteins: novel approaches to the study of the mechanisms of physiological and pathological processes in living systems" was successfully completed. The project focused on the development and use of new approaches to the study of the molecular mechanisms of biological processes and targeted effects on living systems using the latest generation of fluorescent markers. Laboratory of fluorescence bioimaging successfully works, has the world level, and covers almost all the main areas of biomedical researches in the field of fluorescent proteins - the development of technologies of monitoring living tumor cells using fluorescence microscopy and tomography; research opportunities of genetically encoded photosensitizers for targeted destroying cancer cells using cell lines and laboratory animals’ models; analysis of the dynamics of generation of reactive oxygen species in tumors using genetically encoded sensors of hydrogen peroxide, the development of methods of delivery of genetically encoded markers, sensors and photosensitizers to tumor cells in laboratory animals.

The laboratory is equipped with the unique equipment: the first in Russia system of molecular imaging IVIS Spectrum (Caliper Life Science, USA), a diffusion fluorescent scanner designed and developed in IAP RAS (Russia), the only in Russia a fluorescence microscope STORM (Nikon) with ultra-high resolution (of the order 20 nm). Address lab site: http://www.niipfm.nizhgma.ru/bioimidjing/.

 

The theme of megagrant was further developed and realized in two RSF projects:

RSF Project: 14-15-00646 Study of energy metabolism in cancer cells using genetically encoded pH-indicators and fluorescence bioimaging 

The project aims to study the features of energy metabolism of tumor cells in vitro and in vivo models using fluorescence bioimaging techniques.

RSF project (researches performed by the laboratories) 14-25-00129. Development of advanced approaches to the studies of the mechanisms of action of anticancer drugs and early tumor response to the treatment based on optical and molecular technologies 

The project aims to develop methods of analysis of early-response of tumor to drugs for pre-clinical studies.

 

2 PhD theses were defended on the theme of megagrant. 5 postgraduate students continue to develop the theme of megagrant.

In 2013 NNSMA won the third megagrant competition. A professor of the University of Toronto Alex Vitkin became a leading scientist and created another scientific Laboratory of optical coherence tomography. Established infrastructure for megagrant of Lukyanova SA (SPF vivarium, imaging systems) is effectively used for realization of megagrant of A. Vitkin. In 2013 Institute of Biomedical Technologies (http://www.niibmt.nizhgma.ru/) was established on the basis of two laboratories of megagrants. Nowadays Institute of Biomedical Technologies is a perspective, well-organized and working scientific institute, where students, postgraduate students and young researchers work on probation, work and gain professional skills. The Institute is funded by received grants and programs in the field of biophotonics, experimental oncology and regenerative medicine.